Analytical Techniques
Q. If a compound has a molar absorptivity of 200 L/(mol·cm) and a concentration of 0.01 mol/L, what is the absorbance at a path length of 1 cm?
A.
0.2
B.
2
C.
20
D.
0.02
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Solution
Using Beer-Lambert Law (A = εlc), A = 200 L/(mol·cm) * 0.01 mol/L * 1 cm = 2.
Correct Answer: A — 0.2
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Q. If a sample absorbs light at 250 nm with an absorbance of 0.5, what is the transmittance?
A.
50%
B.
25%
C.
75%
D.
100%
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Solution
Transmittance (T) can be calculated using the formula T = 10^(-A). For A = 0.5, T = 10^(-0.5) = 0.316, which is approximately 50%.
Correct Answer: A — 50%
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Q. If a solution has a concentration of 0.1 M and a path length of 1 cm, what is the absorbance if ε = 200 L/(mol·cm)?
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Solution
Using Beer-Lambert Law, A = εcl = 200 L/(mol·cm) * 0.1 mol/L * 1 cm = 20.
Correct Answer: C — 2
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Q. In a back titration, what is the first step?
A.
Add excess titrant to the analyte
B.
Measure the pH of the solution
C.
Dilute the analyte solution
D.
Add a color indicator
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Solution
In a back titration, the first step is to add an excess of titrant to the analyte, followed by titrating the unreacted titrant.
Correct Answer: A — Add excess titrant to the analyte
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Q. In a flame test, which color indicates the presence of sodium ions?
A.
Green
B.
Red
C.
Yellow
D.
Blue
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Solution
Sodium ions produce a characteristic bright yellow color when subjected to a flame test.
Correct Answer: C — Yellow
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Q. In a qualitative analysis using paper chromatography, which factor primarily affects the separation of ions?
A.
Temperature
B.
Solvent polarity
C.
pH of the solution
D.
Ion concentration
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Solution
The solvent polarity is the primary factor affecting the separation of ions in paper chromatography, as it influences the interaction between the stationary phase and the ions.
Correct Answer: B — Solvent polarity
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Q. In a qualitative analysis using paper chromatography, which factor primarily affects the Rf value of a substance?
A.
Solvent polarity
B.
Temperature
C.
pH of the solution
D.
Molecular weight
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Solution
The Rf value is primarily affected by the polarity of the solvent used in paper chromatography, as it influences the interaction between the stationary phase and the analyte.
Correct Answer: A — Solvent polarity
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Q. In a redox reaction, what happens to the substance that is oxidized?
A.
It gains electrons.
B.
It loses electrons.
C.
It is reduced.
D.
It remains unchanged.
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Solution
The substance that is oxidized loses electrons during a redox reaction.
Correct Answer: B — It loses electrons.
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Q. In a redox titration, what is being measured?
A.
The concentration of oxidizing or reducing agents
B.
The temperature of the solution
C.
The volume of gas produced
D.
The solubility of the reactants
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Solution
In a redox titration, the concentration of oxidizing or reducing agents is measured by the amount of titrant required to reach the endpoint.
Correct Answer: A — The concentration of oxidizing or reducing agents
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Q. In a strong acid-strong base titration, what is the expected pH at the equivalence point?
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Solution
At the equivalence point of a strong acid-strong base titration, the pH is expected to be 7 due to the complete neutralization of the acid and base.
Correct Answer: A — 7
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Q. In a titration curve, what does a steep slope indicate?
A.
A weak acid or base
B.
A strong acid or base
C.
A neutral solution
D.
A buffer solution
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Solution
A steep slope in a titration curve indicates a strong acid or base, where the pH changes rapidly with the addition of titrant.
Correct Answer: B — A strong acid or base
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Q. In a titration curve, what does the steepest slope indicate?
A.
The equivalence point
B.
The endpoint
C.
The initial pH
D.
The final pH
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Solution
The steepest slope in a titration curve indicates the equivalence point, where the amount of titrant added is stoichiometrically equivalent to the analyte.
Correct Answer: A — The equivalence point
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Q. In a titration involving a strong acid and a strong base, what is the expected pH at the equivalence point?
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Solution
At the equivalence point of a titration between a strong acid and a strong base, the pH is expected to be 7, indicating a neutral solution.
Correct Answer: A — 7
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Q. In a titration to determine the concentration of chloride ions, which indicator is typically used?
A.
Phenolphthalein
B.
Methyl Orange
C.
Silver Nitrate
D.
Chromate
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Solution
Chromate is used as an indicator in titrations involving chloride ions, particularly when using silver nitrate as the titrant.
Correct Answer: D — Chromate
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Q. In a titration to determine the presence of acetic acid, which indicator would be most appropriate?
A.
Phenolphthalein
B.
Methyl orange
C.
Bromothymol blue
D.
Litmus
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Solution
Phenolphthalein is the most appropriate indicator for titrating weak acids like acetic acid against strong bases, as it changes color at the relevant pH range.
Correct Answer: A — Phenolphthalein
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Q. In a titration to determine the presence of chloride ions, which indicator is most suitable?
A.
Phenolphthalein
B.
Methyl orange
C.
Silver nitrate
D.
Chromate
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Solution
Chromate is used as an indicator in titrations involving chloride ions, as it forms a distinct color change when chloride ions are precipitated.
Correct Answer: D — Chromate
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Q. In a titration to determine the presence of chloride ions, which indicator is typically used?
A.
Phenolphthalein
B.
Methyl Orange
C.
Silver Nitrate
D.
Potassium Chromate
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Solution
Potassium Chromate is used as an indicator in titrations involving silver nitrate to detect chloride ions, forming a red precipitate of silver chromate.
Correct Answer: D — Potassium Chromate
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Q. In a titration to identify chloride ions, which indicator is commonly used?
A.
Phenolphthalein
B.
Methyl orange
C.
Silver nitrate
D.
Chromate
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Solution
Chromate is used as an indicator in titrations involving chloride ions, as it forms a colored precipitate with silver ions.
Correct Answer: D — Chromate
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Q. In a titration, what does the term 'analyte' refer to?
A.
The solution of known concentration
B.
The substance being analyzed
C.
The indicator used
D.
The equipment used
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Solution
The analyte is the substance whose concentration is being determined in the titration process.
Correct Answer: B — The substance being analyzed
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Q. In a titration, what is the endpoint?
A.
The point where the titrant is added
B.
The point where the reaction is complete
C.
The point where the solution changes color
D.
The point where the pH is neutral
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Solution
The endpoint of a titration is the point at which the reaction between the titrant and the analyte is complete, often indicated by a color change.
Correct Answer: B — The point where the reaction is complete
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Q. In a UV-Vis spectrum, what does a higher absorbance value indicate?
A.
Lower concentration of the analyte
B.
Higher concentration of the analyte
C.
Presence of impurities
D.
Increased solvent interference
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Solution
A higher absorbance value in a UV-Vis spectrum indicates a higher concentration of the analyte, according to Beer-Lambert law.
Correct Answer: B — Higher concentration of the analyte
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Q. In a UV-Vis spectrum, what does a peak at 260 nm typically indicate?
A.
Proteins
B.
Nucleic acids
C.
Lipids
D.
Carbohydrates
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Solution
A peak at 260 nm is characteristic of nucleic acids, particularly DNA and RNA.
Correct Answer: B — Nucleic acids
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Q. In a weak acid-strong base titration, what is the pH at the equivalence point?
A.
Less than 7
B.
Equal to 7
C.
Greater than 7
D.
Cannot be determined
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Solution
In a weak acid-strong base titration, the pH at the equivalence point is greater than 7 due to the formation of a weak conjugate base.
Correct Answer: C — Greater than 7
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Q. In electrophoresis, what determines the direction of ion movement?
A.
Molecular weight
B.
Charge of the ion
C.
Solubility
D.
Temperature
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Solution
The direction of ion movement in electrophoresis is determined by the charge of the ion; cations move towards the cathode and anions towards the anode.
Correct Answer: B — Charge of the ion
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Q. In electrophoresis, what factor primarily influences the migration speed of ions?
A.
Ion concentration
B.
Electric field strength
C.
Temperature of the buffer
D.
Size of the gel pores
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Solution
The migration speed of ions in electrophoresis is primarily influenced by the strength of the electric field applied.
Correct Answer: B — Electric field strength
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Q. In electrophoresis, what property of ions is primarily utilized for separation?
A.
Mass
B.
Charge
C.
Solubility
D.
Size
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Solution
Electrophoresis separates ions based on their charge, allowing them to migrate in an electric field.
Correct Answer: B — Charge
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Q. In electrophoresis, what property of ions primarily determines their movement?
A.
Mass
B.
Charge
C.
Size
D.
Solubility
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Solution
The movement of ions in electrophoresis is primarily determined by their charge; positively charged ions move towards the cathode.
Correct Answer: B — Charge
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Q. In flame tests, which color indicates the presence of sodium ions?
A.
Green
B.
Red
C.
Yellow
D.
Blue
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Solution
Sodium ions produce a bright yellow flame when subjected to a flame test.
Correct Answer: C — Yellow
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Q. In flame tests, which color is produced by the presence of sodium ions?
A.
Green
B.
Red
C.
Yellow
D.
Blue
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Solution
Sodium ions produce a characteristic bright yellow color in flame tests.
Correct Answer: C — Yellow
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Q. In gas chromatography, what is the role of the stationary phase?
A.
To vaporize the sample
B.
To separate components based on volatility
C.
To provide a surface for interaction with analytes
D.
To detect the components
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Solution
The stationary phase in gas chromatography provides a surface for interaction with the analytes, allowing for separation based on their different affinities.
Correct Answer: C — To provide a surface for interaction with analytes
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