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In a UV-Vis spectrum, what does a peak at 260 nm typically indicate?
In a UV-Vis spectrum, what does a peak at 260 nm typically indicate?
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In a UV-Vis spectrum, what does a peak at 260 nm typically indicate?
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A peak at 260 nm is characteristic of nucleic acids, particularly DNA and RNA.
Questions & Step-by-step Solutions
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Q: In a UV-Vis spectrum, what does a peak at 260 nm typically indicate?
Solution:
A peak at 260 nm is characteristic of nucleic acids, particularly DNA and RNA.
Steps: 5
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Step 1: Understand what a UV-Vis spectrum is. It is a graph that shows how much light is absorbed by a sample at different wavelengths.
Step 2: Know that different substances absorb light at specific wavelengths. This helps identify what is in the sample.
Step 3: Recognize that 260 nm is a specific wavelength in the UV range.
Step 4: Learn that nucleic acids, like DNA and RNA, absorb light strongly at 260 nm.
Step 5: Conclude that if you see a peak at 260 nm in a UV-Vis spectrum, it usually means that nucleic acids are present in the sample.
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