Intro to Spectroscopy (UV-Vis, IR) - Real World Applications
Q. In IR spectroscopy, what type of molecular vibrations are primarily detected?
A.Rotational
B.Translational
C.Stretching and bending
D.Electron transitions
Solution
IR spectroscopy detects molecular vibrations, specifically stretching and bending of bonds, which correspond to different functional groups.
Correct Answer: C — Stretching and bending
Q. What is a common application of IR spectroscopy in environmental analysis?
A.Measuring pH levels
B.Identifying pollutants
C.Determining molecular weight
D.Separating mixtures
Solution
IR spectroscopy is used to identify pollutants in environmental samples by detecting specific functional groups associated with contaminants.
Correct Answer: B — Identifying pollutants
Q. What is the primary application of UV-Vis spectroscopy in the pharmaceutical industry?
A.Determining molecular weight
B.Measuring absorbance of drug solutions
C.Identifying functional groups
D.Separating compounds
Solution
UV-Vis spectroscopy is commonly used to measure the absorbance of drug solutions, which helps in determining the concentration of the drug in a sample.
Correct Answer: B — Measuring absorbance of drug solutions
Q. What type of information can be obtained from the fingerprint region of an IR spectrum?
A.Functional group identification
B.Molecular weight determination
C.Unique molecular structure
D.Concentration analysis
Solution
The fingerprint region of an IR spectrum contains unique absorption patterns that can be used to identify specific molecular structures.
Correct Answer: C — Unique molecular structure
Q. Which modern analytical instrument combines UV-Vis and fluorescence detection?
A.Mass spectrometer
B.HPLC
C.NMR spectrometer
D.FTIR spectrometer
Solution
High-Performance Liquid Chromatography (HPLC) can be equipped with both UV-Vis and fluorescence detectors for enhanced analysis of compounds.
Correct Answer: B — HPLC
Q. Which type of sample preparation is commonly used before performing IR spectroscopy?
A.Dissolving in water
B.KBr pellet method
C.Filtration
D.Evaporation
Solution
The KBr pellet method is a common sample preparation technique for solid samples in IR spectroscopy, allowing for clear spectral analysis.
