Intro to Spectroscopy (UV-Vis, IR) - Numerical Applications

Q. If a compound has a molar absorptivity of 200 L/(mol·cm) and a concentration of 0.01 mol/L, what is the absorbance at a path length of 1 cm?
  • A. 0.2
  • B. 2
  • C. 20
  • D. 0.02
Q. If a sample absorbs light at 250 nm with an absorbance of 0.5, what is the transmittance?
  • A. 50%
  • B. 25%
  • C. 75%
  • D. 100%
Q. If a solution has a concentration of 0.1 M and a path length of 1 cm, what is the absorbance if ε = 200 L/(mol·cm)?
  • A. 20
  • B. 0.2
  • C. 2
  • D. 200
Q. In a UV-Vis spectrum, what does a peak at 260 nm typically indicate?
  • A. Proteins
  • B. Nucleic acids
  • C. Lipids
  • D. Carbohydrates
Q. In IR spectroscopy, which functional group is characterized by a strong absorption around 1700 cm-1?
  • A. Alcohols
  • B. Aldehydes
  • C. Carboxylic acids
  • D. Ketones
Q. In IR spectroscopy, which functional group is indicated by a strong peak around 1700 cm-1?
  • A. Alcohol
  • B. Aldehyde
  • C. Carboxylic Acid
  • D. Carbonyl
Q. In UV-Vis spectroscopy, what does a higher absorbance indicate about the concentration of a sample?
  • A. Lower concentration
  • B. Higher concentration
  • C. No correlation
  • D. Increased path length
Q. What is the Beer-Lambert Law equation?
  • A. A = εcl
  • B. A = c/εl
  • C. A = l/εc
  • D. A = cl/ε
Q. What is the effect of increasing the path length in a UV-Vis spectrophotometer?
  • A. Decreases absorbance
  • B. Increases absorbance
  • C. No effect on absorbance
  • D. Increases wavelength
Q. What is the primary purpose of using a blank in UV-Vis spectroscopy?
  • A. To calibrate the instrument
  • B. To measure the sample absorbance
  • C. To account for solvent effects
  • D. To determine the concentration
Q. What is the primary use of IR spectroscopy in organic chemistry?
  • A. Determining molecular weight
  • B. Identifying functional groups
  • C. Measuring concentration
  • D. Analyzing reaction rates
Q. What is the typical range of wavelengths for infrared (IR) spectroscopy?
  • A. 400-700 nm
  • B. 700-1400 nm
  • C. 1400-4000 nm
  • D. 4000-10000 nm
Q. What is the wavelength range of UV-Vis spectroscopy?
  • A. 100-400 nm
  • B. 400-700 nm
  • C. 700-1000 nm
  • D. 1000-2000 nm
Q. Which of the following is NOT a common application of UV-Vis spectroscopy?
  • A. Determining protein concentration
  • B. Measuring pH
  • C. Analyzing colored compounds
  • D. Monitoring reaction kinetics
Q. Which type of spectroscopy is best suited for identifying functional groups in organic compounds?
  • A. NMR
  • B. UV-Vis
  • C. IR
  • D. Mass Spectrometry
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