Intro to Spectroscopy (UV-Vis, IR) - Numerical Applications
Q. If a compound has a molar absorptivity of 200 L/(mol·cm) and a concentration of 0.01 mol/L, what is the absorbance at a path length of 1 cm?
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A.
0.2
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B.
2
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C.
20
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D.
0.02
Solution
Using Beer-Lambert Law (A = εlc), A = 200 L/(mol·cm) * 0.01 mol/L * 1 cm = 2.
Correct Answer: A — 0.2
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Q. If a sample absorbs light at 250 nm with an absorbance of 0.5, what is the transmittance?
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A.
50%
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B.
25%
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C.
75%
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D.
100%
Solution
Transmittance (T) can be calculated using the formula T = 10^(-A). For A = 0.5, T = 10^(-0.5) = 0.316, which is approximately 50%.
Correct Answer: A — 50%
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Q. If a solution has a concentration of 0.1 M and a path length of 1 cm, what is the absorbance if ε = 200 L/(mol·cm)?
Solution
Using Beer-Lambert Law, A = εcl = 200 L/(mol·cm) * 0.1 mol/L * 1 cm = 20.
Correct Answer: C — 2
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Q. In a UV-Vis spectrum, what does a peak at 260 nm typically indicate?
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A.
Proteins
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B.
Nucleic acids
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C.
Lipids
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D.
Carbohydrates
Solution
A peak at 260 nm is characteristic of nucleic acids, particularly DNA and RNA.
Correct Answer: B — Nucleic acids
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Q. In IR spectroscopy, which functional group is characterized by a strong absorption around 1700 cm-1?
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A.
Alcohols
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B.
Aldehydes
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C.
Carboxylic acids
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D.
Ketones
Solution
Ketones typically show a strong carbonyl (C=O) absorption around 1700 cm-1 in IR spectroscopy.
Correct Answer: D — Ketones
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Q. In IR spectroscopy, which functional group is indicated by a strong peak around 1700 cm-1?
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A.
Alcohol
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B.
Aldehyde
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C.
Carboxylic Acid
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D.
Carbonyl
Solution
A strong peak around 1700 cm-1 in IR spectroscopy is characteristic of the carbonyl (C=O) functional group.
Correct Answer: D — Carbonyl
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Q. In UV-Vis spectroscopy, what does a higher absorbance indicate about the concentration of a sample?
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A.
Lower concentration
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B.
Higher concentration
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C.
No correlation
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D.
Increased path length
Solution
According to Beer-Lambert Law, higher absorbance indicates a higher concentration of the absorbing species.
Correct Answer: B — Higher concentration
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Q. What is the Beer-Lambert Law equation?
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A.
A = εcl
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B.
A = c/εl
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C.
A = l/εc
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D.
A = cl/ε
Solution
The Beer-Lambert Law is expressed as A = εcl, where A is absorbance, ε is molar absorptivity, c is concentration, and l is path length.
Correct Answer: A — A = εcl
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Q. What is the effect of increasing the path length in a UV-Vis spectrophotometer?
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A.
Decreases absorbance
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B.
Increases absorbance
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C.
No effect on absorbance
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D.
Increases wavelength
Solution
Increasing the path length increases the absorbance according to Beer-Lambert Law.
Correct Answer: B — Increases absorbance
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Q. What is the primary purpose of using a blank in UV-Vis spectroscopy?
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A.
To calibrate the instrument
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B.
To measure the sample absorbance
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C.
To account for solvent effects
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D.
To determine the concentration
Solution
A blank is used to account for any absorbance due to the solvent or other components in the sample.
Correct Answer: C — To account for solvent effects
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Q. What is the primary use of IR spectroscopy in organic chemistry?
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A.
Determining molecular weight
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B.
Identifying functional groups
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C.
Measuring concentration
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D.
Analyzing reaction rates
Solution
IR spectroscopy is primarily used to identify functional groups in organic compounds based on their characteristic absorption bands.
Correct Answer: B — Identifying functional groups
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Q. What is the typical range of wavelengths for infrared (IR) spectroscopy?
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A.
400-700 nm
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B.
700-1400 nm
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C.
1400-4000 nm
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D.
4000-10000 nm
Solution
Infrared spectroscopy typically operates in the range of 1400 to 4000 nm.
Correct Answer: C — 1400-4000 nm
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Q. What is the wavelength range of UV-Vis spectroscopy?
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A.
100-400 nm
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B.
400-700 nm
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C.
700-1000 nm
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D.
1000-2000 nm
Solution
UV-Vis spectroscopy typically covers the wavelength range from 100 to 400 nm for UV and 400 to 700 nm for visible light.
Correct Answer: A — 100-400 nm
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Q. Which of the following is NOT a common application of UV-Vis spectroscopy?
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A.
Determining protein concentration
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B.
Measuring pH
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C.
Analyzing colored compounds
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D.
Monitoring reaction kinetics
Solution
UV-Vis spectroscopy is not typically used for direct pH measurement.
Correct Answer: B — Measuring pH
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Q. Which type of spectroscopy is best suited for identifying functional groups in organic compounds?
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A.
NMR
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B.
UV-Vis
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C.
IR
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D.
Mass Spectrometry
Solution
Infrared (IR) spectroscopy is best suited for identifying functional groups due to its ability to measure molecular vibrations.
Correct Answer: C — IR
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