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What is the primary purpose of using a blank in UV-Vis spectroscopy?
What is the primary purpose of using a blank in UV-Vis spectroscopy?
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Practice Questions
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Q1
What is the primary purpose of using a blank in UV-Vis spectroscopy?
To calibrate the instrument
To measure the sample absorbance
To account for solvent effects
To determine the concentration
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A blank is used to account for any absorbance due to the solvent or other components in the sample.
Questions & Step-by-step Solutions
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Q
Q: What is the primary purpose of using a blank in UV-Vis spectroscopy?
Solution:
A blank is used to account for any absorbance due to the solvent or other components in the sample.
Steps: 5
Show Steps
Step 1: Understand that UV-Vis spectroscopy measures how much light is absorbed by a sample.
Step 2: Recognize that the sample may contain a solvent or other substances that can also absorb light.
Step 3: A blank is a sample that contains only the solvent or components without the substance of interest.
Step 4: By measuring the absorbance of the blank, you can see how much light is absorbed by the solvent or other components.
Step 5: Subtract the absorbance of the blank from the absorbance of the sample to get the true absorbance of the substance you are studying.
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