What is the effect of increasing the path length in a UV-Vis spectrophotometer?
Practice Questions
1 question
Q1
What is the effect of increasing the path length in a UV-Vis spectrophotometer?
Decreases absorbance
Increases absorbance
No effect on absorbance
Increases wavelength
Increasing the path length increases the absorbance according to Beer-Lambert Law.
Questions & Step-by-step Solutions
1 item
Q
Q: What is the effect of increasing the path length in a UV-Vis spectrophotometer?
Solution: Increasing the path length increases the absorbance according to Beer-Lambert Law.
Steps: 5
Step 1: Understand that a UV-Vis spectrophotometer measures how much light is absorbed by a sample.
Step 2: Know that the path length is the distance that light travels through the sample.
Step 3: Learn about Beer-Lambert Law, which states that absorbance (A) is directly proportional to the path length (l) and the concentration (c) of the sample.
Step 4: Realize that if you increase the path length (l), the absorbance (A) will also increase, assuming concentration remains constant.
Step 5: Conclude that a longer path length means more light is absorbed by the sample, leading to higher absorbance readings.
