Q. Which of the following is NOT a step in the process of gene cloning? (2020)
A.
Isolation of DNA
B.
Transformation
C.
Transcription
D.
Insertion of DNA into a vector
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Solution
Transcription is the process of synthesizing RNA from DNA, which is not a step in gene cloning.
Correct Answer: C — Transcription
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Q. Which of the following is NOT a step in the process of recombinant DNA technology? (2020)
A.
Isolation of DNA
B.
Ligation of DNA fragments
C.
Transformation of host cells
D.
Protein synthesis
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Solution
Protein synthesis is not a step in recombinant DNA technology; the process focuses on manipulating DNA rather than directly synthesizing proteins.
Correct Answer: D — Protein synthesis
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Q. Which of the following techniques is used for the separation of DNA fragments? (2019)
A.
PCR
B.
Gel electrophoresis
C.
Cloning
D.
Transformation
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Solution
Gel electrophoresis is a technique used to separate DNA fragments based on their size, allowing for analysis and purification.
Correct Answer: B — Gel electrophoresis
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Q. Which of the following techniques is used to separate DNA fragments based on size? (2022)
A.
PCR
B.
Gel electrophoresis
C.
DNA sequencing
D.
Cloning
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Solution
Gel electrophoresis is a technique used to separate DNA fragments based on their size, allowing for analysis and purification of DNA.
Correct Answer: B — Gel electrophoresis
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Q. Which organism is commonly used as a host for cloning human genes? (2022)
A.
E. coli
B.
Yeast
C.
Mice
D.
All of the above
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Solution
E. coli is the most commonly used host for cloning human genes due to its rapid growth and well-understood genetics, but yeast and mice are also used in specific contexts.
Correct Answer: D — All of the above
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