Q. Which enzyme is commonly used in the process of DNA cloning? (2022)
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A.
DNA polymerase
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B.
Restriction endonuclease
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C.
Ligase
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D.
Reverse transcriptase
Solution
Restriction endonucleases are used to cut DNA at specific sequences, which is essential for DNA cloning.
Correct Answer: B — Restriction endonuclease
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Q. Which enzyme is commonly used in the process of gene cloning? (2018)
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A.
DNA polymerase
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B.
Ligase
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C.
Restriction endonuclease
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D.
RNA polymerase
Solution
Restriction endonucleases are used to cut DNA at specific sequences, facilitating gene cloning.
Correct Answer: C — Restriction endonuclease
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Q. Which of the following biotechnological applications is used in the production of biofuels? (2023)
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A.
Gene therapy
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B.
Microbial fermentation
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C.
Cloning
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D.
Tissue culture
Solution
Microbial fermentation is a key process in the production of biofuels, utilizing microorganisms to convert biomass into energy.
Correct Answer: B — Microbial fermentation
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Q. Which of the following biotechnological applications is used in the treatment of genetic disorders? (2021)
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A.
Gene therapy
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B.
Bioremediation
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C.
Fermentation
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D.
Tissue culture
Solution
Gene therapy involves the introduction of normal genes into cells to correct genetic disorders.
Correct Answer: A — Gene therapy
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Q. Which of the following is a benefit of using genetically modified organisms (GMOs)? (2023)
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A.
Increased crop yield
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B.
Reduced pesticide use
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C.
Enhanced nutritional content
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D.
All of the above
Solution
GMOs can lead to increased crop yield, reduced pesticide use, and enhanced nutritional content.
Correct Answer: D — All of the above
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Q. Which of the following is a biotechnological application in agriculture? (2020)
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A.
Production of insulin
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B.
Genetically modified crops
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C.
Gene therapy
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D.
DNA fingerprinting
Solution
Genetically modified crops are developed to enhance yield, resistance to pests, and tolerance to environmental stresses.
Correct Answer: B — Genetically modified crops
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Q. Which of the following is a biotechnological application in environmental management? (2022)
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A.
Bioremediation
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B.
Gene therapy
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C.
Cloning
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D.
Transgenic animals
Solution
Bioremediation uses microorganisms to degrade environmental contaminants, making it a key application in environmental management.
Correct Answer: A — Bioremediation
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Q. Which of the following is a biotechnological application in medicine? (2019)
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A.
Gene therapy
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B.
Bioremediation
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C.
Biofuels
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D.
Plant breeding
Solution
Gene therapy is a direct application of biotechnology in medicine, aimed at treating genetic disorders.
Correct Answer: A — Gene therapy
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Q. Which of the following is a biotechnological method for waste management? (2022)
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A.
Bioremediation
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B.
Cloning
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C.
PCR
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D.
Gene editing
Solution
Bioremediation uses microorganisms to degrade environmental contaminants, making it a method for waste management.
Correct Answer: A — Bioremediation
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Q. Which of the following is a biotechnological method used for the production of vaccines? (2021)
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A.
Fermentation
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B.
Cloning
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C.
Gene therapy
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D.
DNA sequencing
Solution
Fermentation is a biotechnological method used to produce vaccines by cultivating microorganisms.
Correct Answer: A — Fermentation
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Q. Which of the following is a common application of biotechnology in the food industry? (2020)
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A.
Fermentation
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B.
Genetic modification
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C.
Food preservation
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D.
All of the above
Solution
Biotechnology is widely used in the food industry for fermentation, genetic modification, and food preservation.
Correct Answer: D — All of the above
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Q. Which of the following is a common method for producing transgenic plants? (2022)
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A.
Microinjection
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B.
Agrobacterium-mediated transformation
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C.
Somatic cell nuclear transfer
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D.
CRISPR-Cas9
Solution
Agrobacterium-mediated transformation is a widely used method for producing transgenic plants by transferring genes into plant cells.
Correct Answer: B — Agrobacterium-mediated transformation
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Q. Which of the following is a genetically modified crop? (2019)
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A.
Bt cotton
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B.
Wheat
-
C.
Rice
-
D.
Barley
Solution
Bt cotton is a genetically modified crop that has been engineered to express a bacterial protein that is toxic to certain pests.
Correct Answer: A — Bt cotton
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Q. Which of the following is a method of bioremediation? (2019)
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A.
Using bacteria to clean oil spills
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B.
Using chemical fertilizers
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C.
Planting cover crops
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D.
Using pesticides
Solution
Bioremediation involves using microorganisms, such as bacteria, to degrade environmental contaminants like oil spills.
Correct Answer: A — Using bacteria to clean oil spills
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Q. Which of the following is a method of gene therapy? (2021)
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A.
Using antibiotics
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B.
Inserting a healthy gene into a patient's cells
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C.
Cloning
-
D.
Creating genetically modified crops
Solution
Gene therapy involves inserting a healthy gene into a patient's cells to treat genetic disorders.
Correct Answer: B — Inserting a healthy gene into a patient's cells
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Q. Which of the following is a method of producing insulin using biotechnology? (2018)
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A.
Fermentation
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B.
Hybridization
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C.
Cloning
-
D.
Cross-breeding
Solution
Insulin is produced using fermentation processes involving genetically modified bacteria.
Correct Answer: A — Fermentation
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Q. Which of the following is a method used for cloning in biotechnology? (2019)
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A.
Somatic cell nuclear transfer
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B.
Polymerase chain reaction
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C.
Gel electrophoresis
-
D.
DNA sequencing
Solution
Somatic cell nuclear transfer is a method used for cloning, where the nucleus of a somatic cell is transferred to an egg cell.
Correct Answer: A — Somatic cell nuclear transfer
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Q. Which of the following is a method used for cloning in plants? (2022)
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A.
Micropropagation
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B.
Electroporation
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C.
Transfection
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D.
PCR
Solution
Micropropagation is a method used for cloning plants by growing them from small tissue samples in a controlled environment.
Correct Answer: A — Micropropagation
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Q. Which of the following is a method used for gene cloning? (2023)
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A.
PCR
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B.
Gel electrophoresis
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C.
DNA sequencing
-
D.
All of the above
Solution
PCR (Polymerase Chain Reaction) is a method used for amplifying DNA, which is essential in gene cloning.
Correct Answer: A — PCR
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Q. Which of the following is a method used for the production of genetically modified organisms? (2023)
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A.
Polymerase chain reaction
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B.
Microinjection
-
C.
Gel electrophoresis
-
D.
Cloning
Solution
Microinjection is a method used to introduce foreign DNA into the cells of an organism, facilitating the creation of genetically modified organisms.
Correct Answer: B — Microinjection
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Q. Which of the following is a method used for the production of monoclonal antibodies? (2022)
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A.
Hybridoma technology
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B.
Gene cloning
-
C.
PCR
-
D.
DNA sequencing
Solution
Hybridoma technology is the method used for the production of monoclonal antibodies by fusing myeloma cells with specific immune cells.
Correct Answer: A — Hybridoma technology
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Q. Which of the following is a method used in the production of insulin? (2018)
-
A.
Fermentation
-
B.
Photosynthesis
-
C.
Transcription
-
D.
Translation
Solution
Insulin is produced using fermentation processes involving genetically modified bacteria that can synthesize insulin.
Correct Answer: A — Fermentation
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Q. Which of the following is a potential risk associated with genetically modified crops? (2021)
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A.
Increased biodiversity
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B.
Development of resistant pests
-
C.
Higher nutritional value
-
D.
Lower production costs
Solution
One potential risk of genetically modified crops is the development of resistant pests, which can lead to increased agricultural challenges.
Correct Answer: B — Development of resistant pests
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Q. Which of the following is a potential risk associated with the use of GMOs? (2019)
-
A.
Increased biodiversity
-
B.
Development of resistant pests
-
C.
Higher nutritional value
-
D.
Lower environmental impact
Solution
A potential risk associated with the use of GMOs is the development of resistant pests, which can lead to increased pesticide use.
Correct Answer: B — Development of resistant pests
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Q. Which of the following is a product of biotechnology used in agriculture? (2019)
-
A.
Insulin
-
B.
Bt cotton
-
C.
Human growth hormone
-
D.
Vaccines
Solution
Bt cotton is a genetically modified organism (GMO) that has been engineered to express a bacterial toxin, making it resistant to certain pests.
Correct Answer: B — Bt cotton
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Q. Which of the following is an example of a genetically modified organism used in agriculture? (2023)
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A.
Golden rice
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B.
Organic wheat
-
C.
Traditional maize
-
D.
Heritage tomatoes
Solution
Golden rice is a genetically modified organism enriched with vitamin A to combat malnutrition.
Correct Answer: A — Golden rice
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Q. Which of the following is an example of a genetically modified organism? (2021)
-
A.
Organic tomatoes
-
B.
Bt corn
-
C.
Heritage wheat
-
D.
Wild strawberries
Solution
Bt corn is genetically modified to express a bacterial protein that provides resistance to certain pests.
Correct Answer: B — Bt corn
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Q. Which of the following is NOT a benefit of biotechnology in medicine? (2020)
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A.
Production of monoclonal antibodies
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B.
Development of vaccines
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C.
Creation of synthetic fertilizers
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D.
Gene therapy
Solution
Creation of synthetic fertilizers is not a benefit of biotechnology in medicine; it is related to agricultural biotechnology.
Correct Answer: C — Creation of synthetic fertilizers
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Q. Which of the following is NOT a benefit of genetically modified organisms (GMOs)? (2022)
-
A.
Increased resistance to pests
-
B.
Higher nutritional value
-
C.
Reduced agricultural yield
-
D.
Drought resistance
Solution
Reduced agricultural yield is not a benefit of GMOs; in fact, they are designed to increase yield and resilience.
Correct Answer: C — Reduced agricultural yield
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Q. Which of the following is NOT a benefit of using biotechnology in agriculture? (2022)
-
A.
Reduced pesticide use
-
B.
Increased resistance to diseases
-
C.
Higher production costs
-
D.
Enhanced nutritional content
Solution
Higher production costs are not a benefit of biotechnology; in fact, biotechnology aims to reduce costs.
Correct Answer: C — Higher production costs
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