Sterilization and Disinfection - Applications
Q. What is the minimum effective concentration of alcohol for disinfection?
A.
50%
B.
70%
C.
90%
D.
100%
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Solution
The minimum effective concentration of alcohol for disinfection is typically around 70%, as it allows for better penetration and efficacy.
Correct Answer: B — 70%
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Q. What is the most effective method for sterilizing heat-sensitive medical instruments?
A.
Autoclaving
B.
Ethylene oxide gas
C.
Boiling water
D.
Hydrogen peroxide vapor
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Solution
Ethylene oxide gas is commonly used for sterilizing heat-sensitive medical instruments as it effectively penetrates materials without damaging them.
Correct Answer: B — Ethylene oxide gas
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Q. What is the primary mechanism of action for glutaraldehyde as a disinfectant?
A.
Oxidation of cellular components
B.
Denaturation of proteins
C.
Disruption of nucleic acids
D.
Inhibition of cell wall synthesis
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Solution
Glutaraldehyde acts primarily by denaturing proteins, which leads to the inactivation of microorganisms.
Correct Answer: B — Denaturation of proteins
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Q. What is the primary purpose of sterilization in a clinical setting?
A.
To reduce microbial load
B.
To eliminate all forms of microbial life
C.
To disinfect surfaces
D.
To enhance immune response
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Solution
Sterilization aims to eliminate all forms of microbial life, including bacteria, viruses, fungi, and spores.
Correct Answer: B — To eliminate all forms of microbial life
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Q. What is the role of alcohol in disinfection?
A.
To kill spores
B.
To denature proteins and disrupt membranes
C.
To oxidize cellular components
D.
To inhibit nucleic acid synthesis
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Solution
Alcohol acts as a disinfectant by denaturing proteins and disrupting cellular membranes, making it effective against many microorganisms.
Correct Answer: B — To denature proteins and disrupt membranes
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Q. Which of the following is a common application of disinfection in healthcare?
A.
Sterilizing surgical instruments
B.
Cleaning surfaces in patient rooms
C.
Preparing culture media
D.
Vaccination preparation
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Solution
Disinfection is commonly applied to clean surfaces in patient rooms to reduce the risk of infection.
Correct Answer: B — Cleaning surfaces in patient rooms
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Q. Which of the following is a common application of sterilization in laboratories?
A.
Disinfecting countertops
B.
Sterilizing culture media
C.
Cleaning glassware
D.
Preparing vaccines
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Solution
Sterilization is commonly applied to culture media in laboratories to ensure that no contaminants are present during microbial growth.
Correct Answer: B — Sterilizing culture media
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Q. Which of the following is a limitation of using bleach as a disinfectant?
A.
It is ineffective against viruses
B.
It can corrode surfaces
C.
It is too expensive
D.
It requires a long contact time
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Solution
Bleach can corrode surfaces, which limits its use in certain environments despite its effectiveness against a wide range of pathogens.
Correct Answer: B — It can corrode surfaces
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Q. Which of the following is a method of sterilization that uses high-pressure steam?
A.
Dry heat sterilization
B.
Chemical vapor sterilization
C.
Autoclaving
D.
Radiation sterilization
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Solution
Autoclaving is a method of sterilization that uses high-pressure steam to kill all forms of microbial life.
Correct Answer: C — Autoclaving
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Q. Which of the following methods is NOT typically used for sterilization?
A.
Autoclaving
B.
Dry heat
C.
Chemical disinfection
D.
Filtration
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Solution
Chemical disinfection is used to reduce microbial load but does not guarantee the elimination of all microorganisms, thus it is not a sterilization method.
Correct Answer: C — Chemical disinfection
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