Intro to Spectroscopy (UV-Vis, IR) - Real World Applications
Q. In IR spectroscopy, what type of molecular vibrations are primarily detected?
A.
Rotational
B.
Translational
C.
Stretching and bending
D.
Electron transitions
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Solution
IR spectroscopy detects molecular vibrations, specifically stretching and bending of bonds, which correspond to different functional groups.
Correct Answer: C — Stretching and bending
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Q. In IR spectroscopy, which functional group is typically identified by a strong absorption around 1700 cm-1?
A.
Alcohols
B.
Aldehydes
C.
Carboxylic acids
D.
Ketones
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Solution
A strong absorption around 1700 cm-1 is characteristic of carbonyl groups, which are found in ketones.
Correct Answer: D — Ketones
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Q. What is a common application of IR spectroscopy in environmental analysis?
A.
Measuring pH levels
B.
Identifying pollutants
C.
Determining molecular weight
D.
Separating mixtures
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Solution
IR spectroscopy is used to identify pollutants in environmental samples by detecting specific functional groups associated with contaminants.
Correct Answer: B — Identifying pollutants
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Q. What is a typical application of UV-Vis spectroscopy in food analysis?
A.
Measuring pH
B.
Determining fat content
C.
Analyzing color and pigments
D.
Identifying microbial contamination
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Solution
In food analysis, UV-Vis spectroscopy is often used to analyze color and pigments, which can indicate quality and freshness.
Correct Answer: C — Analyzing color and pigments
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Q. What is the main advantage of using UV-Vis spectroscopy for quantitative analysis?
A.
High sensitivity
B.
Simplicity of the equipment
C.
Ability to analyze solid samples
D.
Rapid analysis time
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Solution
UV-Vis spectroscopy allows for rapid analysis time, making it advantageous for quantitative analysis in various applications.
Correct Answer: D — Rapid analysis time
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Q. What is the primary application of UV-Vis spectroscopy in the pharmaceutical industry?
A.
Determining molecular weight
B.
Measuring absorbance of drug solutions
C.
Identifying functional groups
D.
Separating compounds
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Solution
UV-Vis spectroscopy is commonly used to measure the absorbance of drug solutions, which helps in determining the concentration of the drug in a sample.
Correct Answer: B — Measuring absorbance of drug solutions
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Q. What type of information can be obtained from the fingerprint region of an IR spectrum?
A.
Functional group identification
B.
Molecular weight determination
C.
Unique molecular structure
D.
Concentration analysis
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Solution
The fingerprint region of an IR spectrum contains unique absorption patterns that can be used to identify specific molecular structures.
Correct Answer: C — Unique molecular structure
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Q. Which modern analytical instrument combines UV-Vis and fluorescence detection?
A.
Mass spectrometer
B.
HPLC
C.
NMR spectrometer
D.
FTIR spectrometer
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Solution
High-Performance Liquid Chromatography (HPLC) can be equipped with both UV-Vis and fluorescence detectors for enhanced analysis of compounds.
Correct Answer: B — HPLC
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Q. Which modern analytical instrument is commonly used for both UV-Vis and IR spectroscopy?
A.
Mass spectrometer
B.
Nuclear magnetic resonance
C.
Spectrophotometer
D.
Chromatograph
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Solution
A spectrophotometer is the modern analytical instrument commonly used for both UV-Vis and IR spectroscopy.
Correct Answer: C — Spectrophotometer
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Q. Which of the following compounds would you expect to show a strong absorption in the IR region due to O-H stretching?
A.
Ethanol
B.
Benzene
C.
Hexane
D.
Cyclohexane
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Solution
Ethanol would show a strong absorption in the IR region due to O-H stretching, characteristic of alcohols.
Correct Answer: A — Ethanol
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Q. Which of the following is a common application of IR spectroscopy in environmental science?
A.
Measuring pH levels
B.
Identifying pollutants in air samples
C.
Determining molecular weight
D.
Separating mixtures
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Solution
IR spectroscopy is commonly used to identify pollutants in air samples by analyzing their characteristic absorption spectra.
Correct Answer: B — Identifying pollutants in air samples
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Q. Which type of sample preparation is commonly used before performing IR spectroscopy?
A.
Dissolving in water
B.
KBr pellet method
C.
Filtration
D.
Evaporation
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Solution
The KBr pellet method is a common sample preparation technique for solid samples in IR spectroscopy, allowing for clear spectral analysis.
Correct Answer: B — KBr pellet method
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