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Which technique is used to separate DNA fragments based on size?
Which technique is used to separate DNA fragments based on size?
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Which technique is used to separate DNA fragments based on size?
PCR
Gel electrophoresis
Cloning
DNA sequencing
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Gel electrophoresis is used to separate DNA fragments according to their size, allowing for analysis and comparison.
Questions & Step-by-step Solutions
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Q: Which technique is used to separate DNA fragments based on size?
Solution:
Gel electrophoresis is used to separate DNA fragments according to their size, allowing for analysis and comparison.
Steps: 7
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Step 1: Understand that DNA is made up of different sized fragments.
Step 2: Know that gel electrophoresis is a technique used to separate these DNA fragments.
Step 3: Prepare a gel, usually made of agarose, which acts like a sieve.
Step 4: Load the DNA samples into wells in the gel.
Step 5: Apply an electric current to the gel; DNA fragments will move through the gel.
Step 6: Smaller DNA fragments move faster and travel further than larger ones.
Step 7: After the run is complete, the DNA fragments are separated by size.
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