Which technique is best suited for separating and identifying amino acids in a mixture?
Practice Questions
1 question
Q1
Which technique is best suited for separating and identifying amino acids in a mixture?
Gas chromatography
High-performance liquid chromatography
Thin-layer chromatography
Capillary electrophoresis
Capillary electrophoresis is particularly effective for separating and identifying amino acids due to their charge and size differences.
Questions & Step-by-step Solutions
1 item
Q
Q: Which technique is best suited for separating and identifying amino acids in a mixture?
Solution: Capillary electrophoresis is particularly effective for separating and identifying amino acids due to their charge and size differences.
Steps: 5
Step 1: Understand that amino acids are the building blocks of proteins and can have different charges depending on their pH environment.
Step 2: Learn that capillary electrophoresis is a technique that uses an electric field to separate molecules based on their size and charge.
Step 3: Recognize that in capillary electrophoresis, amino acids will move at different speeds through a gel or liquid medium when an electric current is applied.
Step 4: Realize that this separation allows for the identification of each amino acid based on how far they travel in a given time.
Step 5: Conclude that capillary electrophoresis is the best technique for separating and identifying amino acids in a mixture.
