What is the significance of the 'sticky ends' produced by some restriction enzymes?
Practice Questions
1 question
Q1
What is the significance of the 'sticky ends' produced by some restriction enzymes?
They allow for easier ligation of DNA fragments
They prevent DNA degradation
They enhance PCR efficiency
They facilitate RNA transcription
'Sticky ends' created by certain restriction enzymes allow for easier ligation of DNA fragments by providing complementary overhangs.
Questions & Step-by-step Solutions
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Q
Q: What is the significance of the 'sticky ends' produced by some restriction enzymes?
Solution: 'Sticky ends' created by certain restriction enzymes allow for easier ligation of DNA fragments by providing complementary overhangs.
Steps: 5
Step 1: Understand what restriction enzymes are. They are proteins that cut DNA at specific sequences.
Step 2: Learn about 'sticky ends'. When some restriction enzymes cut DNA, they leave overhanging ends that are not paired with complementary bases.
Step 3: Recognize that these overhangs are called 'sticky ends' because they can easily stick to other DNA fragments that have complementary sequences.
Step 4: Know that this sticking helps in the process of ligation, which is when two pieces of DNA are joined together.
Step 5: Realize that having sticky ends makes it easier to combine different DNA pieces in genetic engineering and cloning.
