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What is the significance of the 'sticky ends' in DNA cloning?
What is the significance of the 'sticky ends' in DNA cloning?
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What is the significance of the 'sticky ends' in DNA cloning?
They prevent DNA degradation
They facilitate the ligation of DNA fragments
They enhance PCR efficiency
They stabilize RNA structures
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'Sticky ends' are overhanging sequences that allow for the easy joining of DNA fragments during cloning.
Questions & Step-by-step Solutions
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Q
Q: What is the significance of the 'sticky ends' in DNA cloning?
Solution:
'Sticky ends' are overhanging sequences that allow for the easy joining of DNA fragments during cloning.
Steps: 6
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Step 1: Understand that DNA is made up of sequences of nucleotides.
Step 2: Learn that during DNA cloning, we want to join different pieces of DNA together.
Step 3: Know that 'sticky ends' are short, single-stranded overhangs at the ends of DNA fragments.
Step 4: Realize that these overhangs can easily pair with complementary sequences on other DNA fragments.
Step 5: Understand that this pairing helps to hold the DNA pieces together securely.
Step 6: Remember that using 'sticky ends' makes it easier and more efficient to join DNA fragments during cloning.
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