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What is the significance of the 'sticky ends' created by restriction enzymes?
What is the significance of the 'sticky ends' created by restriction enzymes?
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What is the significance of the 'sticky ends' created by restriction enzymes?
They stabilize RNA
They allow for the easy joining of DNA fragments
They prevent mutations
They enhance PCR efficiency
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'Sticky ends' are overhanging sequences that facilitate the ligation of DNA fragments during cloning.
Questions & Step-by-step Solutions
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Q: What is the significance of the 'sticky ends' created by restriction enzymes?
Solution:
'Sticky ends' are overhanging sequences that facilitate the ligation of DNA fragments during cloning.
Steps: 5
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Step 1: Understand that restriction enzymes are proteins that cut DNA at specific sequences.
Step 2: When these enzymes cut the DNA, they can create 'sticky ends', which are short, single-stranded overhangs at the ends of the DNA fragments.
Step 3: These sticky ends can easily pair with complementary sticky ends of other DNA fragments.
Step 4: This pairing allows the DNA fragments to stick together, which is important for combining different pieces of DNA.
Step 5: The process of joining these DNA fragments is called ligation, and it is essential in cloning and genetic engineering.
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