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What is the significance of the 'denaturation' step in PCR?
What is the significance of the 'denaturation' step in PCR?
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Q1
What is the significance of the 'denaturation' step in PCR?
To cool the reaction mixture
To separate the DNA strands
To synthesize new DNA strands
To add primers
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Denaturation involves heating the reaction mixture to separate the double-stranded DNA into single strands.
Questions & Step-by-step Solutions
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Q
Q: What is the significance of the 'denaturation' step in PCR?
Solution:
Denaturation involves heating the reaction mixture to separate the double-stranded DNA into single strands.
Steps: 4
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Step 1: Start with a mixture that contains double-stranded DNA.
Step 2: Heat the mixture to a high temperature (usually around 94-98 degrees Celsius).
Step 3: The heat causes the two strands of the DNA to separate, turning them into single strands.
Step 4: This separation is crucial because it allows the next steps of PCR to occur, where new DNA strands can be made.
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