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What is the purpose of using a reference sample in UV-Vis spectroscopy?
What is the purpose of using a reference sample in UV-Vis spectroscopy?
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What is the purpose of using a reference sample in UV-Vis spectroscopy?
To calibrate the instrument
To increase the sensitivity of the measurement
To compare the sample's absorbance against a known standard
To reduce background noise
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A reference sample is used to compare the absorbance of the sample against a known standard, ensuring accurate measurements.
Questions & Step-by-step Solutions
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Q
Q: What is the purpose of using a reference sample in UV-Vis spectroscopy?
Solution:
A reference sample is used to compare the absorbance of the sample against a known standard, ensuring accurate measurements.
Steps: 5
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Step 1: Understand that UV-Vis spectroscopy measures how much light a sample absorbs.
Step 2: Know that a reference sample is a solution that does not contain the substance you are testing.
Step 3: Use the reference sample to set a baseline measurement of light absorption.
Step 4: Compare the absorbance of your actual sample to the reference sample.
Step 5: This comparison helps ensure that your measurements are accurate and reliable.
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