What is the main advantage of high-performance liquid chromatography (HPLC) over traditional liquid chromatography?
Practice Questions
1 question
Q1
What is the main advantage of high-performance liquid chromatography (HPLC) over traditional liquid chromatography?
Higher resolution and speed
Lower cost of operation
Simpler instrumentation
Use of larger sample volumes
HPLC offers higher resolution and speed due to the use of high pressure to push solvents through the column, allowing for better separation of components.
Questions & Step-by-step Solutions
1 item
Q
Q: What is the main advantage of high-performance liquid chromatography (HPLC) over traditional liquid chromatography?
Solution: HPLC offers higher resolution and speed due to the use of high pressure to push solvents through the column, allowing for better separation of components.
Steps: 6
Step 1: Understand that chromatography is a method used to separate different components in a mixture.
Step 2: Learn that traditional liquid chromatography uses gravity to move solvents through a column.
Step 3: Know that high-performance liquid chromatography (HPLC) uses high pressure instead of gravity.
Step 4: Realize that the high pressure in HPLC allows solvents to move faster through the column.
Step 5: Understand that faster movement leads to quicker results and better separation of the components.
Step 6: Conclude that the main advantage of HPLC is its ability to provide higher resolution and speed compared to traditional methods.
