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What is a common method for visualizing DNA after gel electrophoresis?
What is a common method for visualizing DNA after gel electrophoresis?
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Practice Questions
1 question
Q1
What is a common method for visualizing DNA after gel electrophoresis?
Fluorescent microscopy
Ethidium bromide staining
Western blotting
Southern blotting
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Ethidium bromide staining intercalates with DNA and fluoresces under UV light, allowing visualization of DNA bands.
Questions & Step-by-step Solutions
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Q: What is a common method for visualizing DNA after gel electrophoresis?
Solution:
Ethidium bromide staining intercalates with DNA and fluoresces under UV light, allowing visualization of DNA bands.
Steps: 5
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Step 1: After performing gel electrophoresis, the DNA is separated in the gel.
Step 2: Ethidium bromide is added to the gel. This is a special dye that can attach to DNA.
Step 3: The gel is then exposed to ultraviolet (UV) light.
Step 4: When the UV light shines on the gel, the ethidium bromide that is attached to the DNA glows.
Step 5: This glowing allows us to see the DNA bands clearly.
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