In UV-Vis spectroscopy, what is the effect of increasing the path length of the sample cell?
Practice Questions
1 question
Q1
In UV-Vis spectroscopy, what is the effect of increasing the path length of the sample cell?
Decreases absorbance
Increases absorbance
No effect on absorbance
Increases transmittance
Increasing the path length of the sample cell increases the absorbance according to the Beer-Lambert Law, as absorbance is directly proportional to path length.
Questions & Step-by-step Solutions
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Q
Q: In UV-Vis spectroscopy, what is the effect of increasing the path length of the sample cell?
Solution: Increasing the path length of the sample cell increases the absorbance according to the Beer-Lambert Law, as absorbance is directly proportional to path length.
Steps: 6
Step 1: Understand what UV-Vis spectroscopy is. It is a technique used to measure how much light is absorbed by a sample.
Step 2: Learn about the sample cell. This is the container that holds the sample being tested.
Step 3: Know what path length means. Path length is the distance that light travels through the sample in the cell.
Step 4: Familiarize yourself with the Beer-Lambert Law. This law states that absorbance (how much light is absorbed) is directly proportional to the path length of the sample.
Step 5: Realize that if you increase the path length, the light has to travel through more of the sample.
Step 6: Understand that as the path length increases, more light is absorbed by the sample, leading to higher absorbance readings.
