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In UV-Vis spectroscopy, what does a peak at 260 nm typically indicate?
In UV-Vis spectroscopy, what does a peak at 260 nm typically indicate?
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In UV-Vis spectroscopy, what does a peak at 260 nm typically indicate?
Presence of proteins
Presence of nucleic acids
Presence of carbohydrates
Presence of lipids
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A peak at 260 nm in UV-Vis spectroscopy typically indicates the presence of nucleic acids, as they absorb light at this wavelength.
Questions & Step-by-step Solutions
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Q
Q: In UV-Vis spectroscopy, what does a peak at 260 nm typically indicate?
Solution:
A peak at 260 nm in UV-Vis spectroscopy typically indicates the presence of nucleic acids, as they absorb light at this wavelength.
Steps: 5
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Step 1: Understand what UV-Vis spectroscopy is. It is a technique used to measure how much light is absorbed by a sample at different wavelengths.
Step 2: Know that different substances absorb light at specific wavelengths. This helps identify what is in the sample.
Step 3: Recognize that nucleic acids, like DNA and RNA, have a characteristic absorption peak.
Step 4: Learn that nucleic acids typically absorb light at a wavelength of 260 nm.
Step 5: Conclude that if you see a peak at 260 nm in your UV-Vis spectrum, it usually means that nucleic acids are present in the sample.
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