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In UV-Vis spectroscopy, what does a higher absorbance value indicate?
In UV-Vis spectroscopy, what does a higher absorbance value indicate?
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In UV-Vis spectroscopy, what does a higher absorbance value indicate?
Lower concentration of the analyte
Higher concentration of the analyte
Higher wavelength of light
Lower path length of the sample
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A higher absorbance value indicates a higher concentration of the analyte, according to Beer-Lambert law.
Questions & Step-by-step Solutions
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Q
Q: In UV-Vis spectroscopy, what does a higher absorbance value indicate?
Solution:
A higher absorbance value indicates a higher concentration of the analyte, according to Beer-Lambert law.
Steps: 5
Show Steps
Step 1: Understand what absorbance means. Absorbance is a measure of how much light is absorbed by a sample.
Step 2: Know that in UV-Vis spectroscopy, we shine light through a sample and measure how much light comes out.
Step 3: Remember that the Beer-Lambert law relates absorbance to concentration. It states that absorbance increases with concentration.
Step 4: Realize that a higher absorbance value means that more light is being absorbed by the sample.
Step 5: Conclude that if more light is absorbed, it indicates that there is a higher concentration of the substance (analyte) in the sample.
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