What is the purpose of using a buffer solution in electrophoresis?
Practice Questions
Q1
What is the purpose of using a buffer solution in electrophoresis?
To increase the conductivity of the solution
To maintain a constant pH during the separation
To enhance the visibility of the bands
To prevent overheating of the gel
Questions & Step-by-Step Solutions
What is the purpose of using a buffer solution in electrophoresis?
Step 1: Understand that electrophoresis is a technique used to separate charged molecules, like DNA or proteins, based on their size and charge.
Step 2: Know that the separation process happens in a gel or liquid medium where an electric current is applied.
Step 3: Realize that the pH level of the medium can affect how the charged molecules behave during separation.
Step 4: Learn that a buffer solution is a special type of solution that helps keep the pH level stable.
Step 5: Understand that by maintaining a stable pH, the buffer solution ensures that the charged molecules move correctly and separate properly during electrophoresis.
