Which technique is best suited for separating and identifying amino acids in a m

Practice Questions

Q1
Which technique is best suited for separating and identifying amino acids in a mixture?
  1. Gas chromatography
  2. High-performance liquid chromatography
  3. Thin-layer chromatography
  4. Capillary electrophoresis

Questions & Step-by-Step Solutions

Which technique is best suited for separating and identifying amino acids in a mixture?
  • Step 1: Understand that amino acids are the building blocks of proteins and can have different charges depending on their pH environment.
  • Step 2: Learn that capillary electrophoresis is a technique that uses an electric field to separate molecules based on their size and charge.
  • Step 3: Recognize that in capillary electrophoresis, amino acids will move at different speeds through a gel or liquid medium when an electric current is applied.
  • Step 4: Realize that this separation allows for the identification of each amino acid based on how far they travel in a given time.
  • Step 5: Conclude that capillary electrophoresis is the best technique for separating and identifying amino acids in a mixture.
No concepts available.
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