In UV-Vis spectroscopy, what does a higher absorbance value indicate?

Practice Questions

Q1
In UV-Vis spectroscopy, what does a higher absorbance value indicate?
  1. Lower concentration of the analyte
  2. Higher concentration of the analyte
  3. Higher wavelength of light
  4. Lower path length of the sample

Questions & Step-by-Step Solutions

In UV-Vis spectroscopy, what does a higher absorbance value indicate?
  • Step 1: Understand what absorbance means. Absorbance is a measure of how much light is absorbed by a sample.
  • Step 2: Know that in UV-Vis spectroscopy, we shine light through a sample and measure how much light comes out.
  • Step 3: Remember that the Beer-Lambert law relates absorbance to concentration. It states that absorbance increases with concentration.
  • Step 4: Realize that a higher absorbance value means that more light is being absorbed by the sample.
  • Step 5: Conclude that if more light is absorbed, it indicates that there is a higher concentration of the substance (analyte) in the sample.
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