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What is the primary purpose of using a blank in UV-Vis spectroscopy?

Practice Questions

Q1
What is the primary purpose of using a blank in UV-Vis spectroscopy?
  1. To calibrate the instrument
  2. To measure the sample absorbance
  3. To account for solvent effects
  4. To determine the concentration

Questions & Step-by-Step Solutions

What is the primary purpose of using a blank in UV-Vis spectroscopy?
  • Step 1: Understand that UV-Vis spectroscopy measures how much light is absorbed by a sample.
  • Step 2: Recognize that the sample may contain a solvent or other substances that can also absorb light.
  • Step 3: A blank is a sample that contains only the solvent or components without the substance of interest.
  • Step 4: By measuring the absorbance of the blank, you can see how much light is absorbed by the solvent or other components.
  • Step 5: Subtract the absorbance of the blank from the absorbance of the sample to get the true absorbance of the substance you are studying.
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