In UV-Vis spectroscopy, what is the effect of increasing the path length of the
Practice Questions
Q1
In UV-Vis spectroscopy, what is the effect of increasing the path length of the sample cell?
Decreases absorbance
Increases absorbance
No effect on absorbance
Increases transmittance
Questions & Step-by-Step Solutions
In UV-Vis spectroscopy, what is the effect of increasing the path length of the sample cell?
Step 1: Understand what UV-Vis spectroscopy is. It is a technique used to measure how much light is absorbed by a sample.
Step 2: Learn about the sample cell. This is the container that holds the sample being tested.
Step 3: Know what path length means. Path length is the distance that light travels through the sample in the cell.
Step 4: Familiarize yourself with the Beer-Lambert Law. This law states that absorbance (how much light is absorbed) is directly proportional to the path length of the sample.
Step 5: Realize that if you increase the path length, the light has to travel through more of the sample.
Step 6: Understand that as the path length increases, more light is absorbed by the sample, leading to higher absorbance readings.
