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What is the purpose of using a reference sample in UV-Vis spectroscopy?

Practice Questions

Q1
What is the purpose of using a reference sample in UV-Vis spectroscopy?
  1. To calibrate the instrument
  2. To increase the sensitivity of the measurement
  3. To compare the sample's absorbance against a known standard
  4. To reduce background noise

Questions & Step-by-Step Solutions

What is the purpose of using a reference sample in UV-Vis spectroscopy?
  • Step 1: Understand that UV-Vis spectroscopy measures how much light a sample absorbs.
  • Step 2: Know that a reference sample is a solution that does not contain the substance you are testing.
  • Step 3: Use the reference sample to set a baseline measurement of light absorption.
  • Step 4: Compare the absorbance of your actual sample to the reference sample.
  • Step 5: This comparison helps ensure that your measurements are accurate and reliable.
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