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What is the significance of the Beer-Lambert Law in UV-Vis spectroscopy?

Practice Questions

Q1
What is the significance of the Beer-Lambert Law in UV-Vis spectroscopy?
  1. It relates absorbance to concentration and path length.
  2. It describes the scattering of light in a medium.
  3. It explains the emission spectra of compounds.
  4. It determines the molecular weight of a substance.

Questions & Step-by-Step Solutions

What is the significance of the Beer-Lambert Law in UV-Vis spectroscopy?
  • Step 1: Understand that UV-Vis spectroscopy is a technique used to measure how much light is absorbed by a sample.
  • Step 2: Learn that the Beer-Lambert Law is a formula that helps us understand this absorption.
  • Step 3: Know that the law states that absorbance (how much light is absorbed) is directly related to two things: the concentration of the substance in the sample and the distance the light travels through the sample (path length).
  • Step 4: Realize that if you increase the concentration of the substance or the path length, the absorbance will increase.
  • Step 5: Use this law to determine the concentration of unknown samples by measuring their absorbance.
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