What is the purpose of using a blank in UV-Vis spectroscopy?

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Question: What is the purpose of using a blank in UV-Vis spectroscopy?

Options:

Correct Answer: To account for solvent absorbance

Solution:

A blank is used to account for the absorbance of the solvent, ensuring that only the sample\'s absorbance is measured.

What is the purpose of using a blank in UV-Vis spectroscopy?

What is the purpose of using a blank in UV-Vis spectroscopy?

Step 1: Understand that UV-Vis spectroscopy measures how much light is absorbed by a sample.
Step 2: Know that the sample is usually dissolved in a solvent (like water or ethanol).
Step 3: Realize that the solvent can also absorb some light, which can affect the results.
Step 4: Use a blank, which is just the solvent without the sample, to measure how much light the solvent absorbs.
Step 5: Subtract the absorbance of the blank from the absorbance of the sample to get the true absorbance of the sample only.

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