What is the purpose of using a blank in UV-Vis spectroscopy?

Practice Questions

Q1
What is the purpose of using a blank in UV-Vis spectroscopy?
  1. To calibrate the instrument
  2. To measure the sample concentration
  3. To account for solvent absorbance
  4. To enhance signal detection

Questions & Step-by-Step Solutions

What is the purpose of using a blank in UV-Vis spectroscopy?
  • Step 1: Understand that UV-Vis spectroscopy measures how much light is absorbed by a sample.
  • Step 2: Know that the sample is usually dissolved in a solvent (like water or ethanol).
  • Step 3: Realize that the solvent can also absorb some light, which can affect the results.
  • Step 4: Use a blank, which is just the solvent without the sample, to measure how much light the solvent absorbs.
  • Step 5: Subtract the absorbance of the blank from the absorbance of the sample to get the true absorbance of the sample only.
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