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A culture shows a growth of 1,200 colonies after incubation. If the dilution fac

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Question: A culture shows a growth of 1,200 colonies after incubation. If the dilution factor was 1:100, what was the original concentration of bacteria in the sample?

Options:

  1. 120,000 CFU/mL
  2. 12,000 CFU/mL
  3. 1,200 CFU/mL
  4. 120 CFU/mL

Correct Answer: 120,000 CFU/mL

Solution: 

To find the original concentration, multiply the number of colonies by the dilution factor: 1,200 colonies * 100 = 120,000 CFU/mL.

A culture shows a growth of 1,200 colonies after incubation. If the dilution fac

Practice Questions

Q1
A culture shows a growth of 1,200 colonies after incubation. If the dilution factor was 1:100, what was the original concentration of bacteria in the sample?
  1. 120,000 CFU/mL
  2. 12,000 CFU/mL
  3. 1,200 CFU/mL
  4. 120 CFU/mL

Questions & Step-by-Step Solutions

A culture shows a growth of 1,200 colonies after incubation. If the dilution factor was 1:100, what was the original concentration of bacteria in the sample?
  • Step 1: Understand that the number of colonies (1,200) represents the number of bacteria that grew from a diluted sample.
  • Step 2: Recognize that the dilution factor (1:100) means that the original sample was diluted 100 times.
  • Step 3: To find the original concentration of bacteria, you need to multiply the number of colonies by the dilution factor.
  • Step 4: Perform the multiplication: 1,200 colonies * 100 = 120,000.
  • Step 5: The result (120,000) represents the original concentration of bacteria in the sample, measured in CFU/mL (colony-forming units per milliliter).
  • Dilution Factor – Understanding how dilution affects the concentration of bacteria in a sample.
  • Colony Forming Units (CFU) – Interpreting the number of colonies as a measure of viable bacterial cells.
  • Basic Arithmetic in Biology – Applying multiplication to calculate original concentrations from diluted samples.
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