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What is the significance of the 'sticky ends' in recombinant DNA technology?

Practice Questions

Q1
What is the significance of the 'sticky ends' in recombinant DNA technology?
  1. They help in DNA replication
  2. They facilitate the ligation of DNA fragments
  3. They are used for PCR amplification
  4. They stabilize the DNA structure

Questions & Step-by-Step Solutions

What is the significance of the 'sticky ends' in recombinant DNA technology?
  • Step 1: Understand that DNA is made up of sequences of nucleotides.
  • Step 2: Learn that when DNA is cut by certain enzymes, it can create 'sticky ends' which are short, single-stranded overhangs.
  • Step 3: Recognize that these sticky ends can easily pair with complementary sequences of DNA.
  • Step 4: Realize that this pairing allows for the joining of different DNA fragments, which is essential in recombinant DNA technology.
  • Step 5: Conclude that 'sticky ends' make it easier to insert new genes into DNA, helping scientists create modified organisms.
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