What is the significance of the 'sticky ends' produced by some restriction enzym

Practice Questions

Q1
What is the significance of the 'sticky ends' produced by some restriction enzymes?
  1. They allow for easier ligation of DNA fragments
  2. They prevent DNA degradation
  3. They enhance PCR efficiency
  4. They facilitate RNA transcription

Questions & Step-by-Step Solutions

What is the significance of the 'sticky ends' produced by some restriction enzymes?
Correct Answer: Sticky ends help in joining DNA fragments easily.
  • Step 1: Understand what restriction enzymes are. They are proteins that cut DNA at specific sequences.
  • Step 2: Learn about 'sticky ends'. When some restriction enzymes cut DNA, they leave overhanging ends that are not paired with complementary bases.
  • Step 3: Recognize that these overhangs are called 'sticky ends' because they can easily stick to other DNA fragments that have complementary sequences.
  • Step 4: Know that this sticking helps in the process of ligation, which is when two pieces of DNA are joined together.
  • Step 5: Realize that having sticky ends makes it easier to combine different DNA pieces in genetic engineering and cloning.
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