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Which technique is used to separate DNA fragments based on size?

Practice Questions

Q1
Which technique is used to separate DNA fragments based on size?
  1. PCR
  2. Gel electrophoresis
  3. Cloning
  4. DNA sequencing

Questions & Step-by-Step Solutions

Which technique is used to separate DNA fragments based on size?
  • Step 1: Understand that DNA is made up of different sized fragments.
  • Step 2: Know that gel electrophoresis is a technique used to separate these DNA fragments.
  • Step 3: Prepare a gel, usually made of agarose, which acts like a sieve.
  • Step 4: Load the DNA samples into wells in the gel.
  • Step 5: Apply an electric current to the gel; DNA fragments will move through the gel.
  • Step 6: Smaller DNA fragments move faster and travel further than larger ones.
  • Step 7: After the run is complete, the DNA fragments are separated by size.
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