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What is the significance of the 'denaturation' step in PCR?
Practice Questions
Q1
What is the significance of the 'denaturation' step in PCR?
To cool the reaction mixture
To separate the DNA strands
To synthesize new DNA strands
To add primers
Questions & Step-by-Step Solutions
What is the significance of the 'denaturation' step in PCR?
Steps
Concepts
Step 1: Start with a mixture that contains double-stranded DNA.
Step 2: Heat the mixture to a high temperature (usually around 94-98 degrees Celsius).
Step 3: The heat causes the two strands of the DNA to separate, turning them into single strands.
Step 4: This separation is crucial because it allows the next steps of PCR to occur, where new DNA strands can be made.
No concepts available.
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