What is the purpose of using reverse transcriptase in molecular biology?

Practice Questions

Q1
What is the purpose of using reverse transcriptase in molecular biology?
  1. To synthesize DNA from RNA
  2. To amplify DNA
  3. To cut DNA at specific sites
  4. To clone DNA

Questions & Step-by-Step Solutions

What is the purpose of using reverse transcriptase in molecular biology?
Correct Answer: Reverse transcriptase is used to synthesize complementary DNA (cDNA) from an RNA template.
  • Step 1: Understand that reverse transcriptase is an enzyme.
  • Step 2: Know that this enzyme helps in converting RNA into DNA.
  • Step 3: Recognize that the DNA made from RNA is called complementary DNA (cDNA).
  • Step 4: Realize that scientists use cDNA for various experiments, like studying genes.
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