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Which technique is used to separate DNA fragments by size?

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Question: Which technique is used to separate DNA fragments by size?

Options:

  1. Gel electrophoresis
  2. PCR
  3. Cloning
  4. Sanger sequencing

Correct Answer: Gel electrophoresis

Solution: 

Gel electrophoresis is a method used to separate DNA fragments based on their size and charge.

Which technique is used to separate DNA fragments by size?

Practice Questions

Q1
Which technique is used to separate DNA fragments by size?
  1. Gel electrophoresis
  2. PCR
  3. Cloning
  4. Sanger sequencing

Questions & Step-by-Step Solutions

Which technique is used to separate DNA fragments by size?
Correct Answer: Gel electrophoresis
  • Step 1: Prepare a gel, usually made of agarose, which will act as a filter for the DNA fragments.
  • Step 2: Mix the DNA samples with a loading dye to make them visible and easier to handle.
  • Step 3: Load the DNA samples into wells (small holes) in the gel.
  • Step 4: Connect the gel to an electric current; DNA is negatively charged and will move towards the positive end.
  • Step 5: As the electric current runs, smaller DNA fragments will move faster and farther through the gel than larger ones.
  • Step 6: After a set amount of time, turn off the current and visualize the separated DNA fragments, usually by staining the gel.
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