What is the significance of the 'sticky ends' in DNA cloning?

Practice Questions

Q1
What is the significance of the 'sticky ends' in DNA cloning?
  1. They prevent DNA degradation
  2. They facilitate the ligation of DNA fragments
  3. They enhance PCR efficiency
  4. They stabilize RNA structures

Questions & Step-by-Step Solutions

What is the significance of the 'sticky ends' in DNA cloning?
Correct Answer: Sticky ends help in joining DNA fragments easily.
  • Step 1: Understand that DNA is made up of sequences of nucleotides.
  • Step 2: Learn that during DNA cloning, we want to join different pieces of DNA together.
  • Step 3: Know that 'sticky ends' are short, single-stranded overhangs at the ends of DNA fragments.
  • Step 4: Realize that these overhangs can easily pair with complementary sequences on other DNA fragments.
  • Step 5: Understand that this pairing helps to hold the DNA pieces together securely.
  • Step 6: Remember that using 'sticky ends' makes it easier and more efficient to join DNA fragments during cloning.
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