What is the significance of the 'sticky ends' created by restriction enzymes?
Correct Answer: Sticky ends help in joining DNA fragments easily.
- Step 1: Understand that restriction enzymes are proteins that cut DNA at specific sequences.
- Step 2: When these enzymes cut the DNA, they can create 'sticky ends', which are short, single-stranded overhangs at the ends of the DNA fragments.
- Step 3: These sticky ends can easily pair with complementary sticky ends of other DNA fragments.
- Step 4: This pairing allows the DNA fragments to stick together, which is important for combining different pieces of DNA.
- Step 5: The process of joining these DNA fragments is called ligation, and it is essential in cloning and genetic engineering.
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