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If a biotechnologist has 200 mg of a plasmid DNA and needs to prepare a 1:10 dil

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Question: If a biotechnologist has 200 mg of a plasmid DNA and needs to prepare a 1:10 dilution, how much diluent should be added?

Options:

  1. 180 mg
  2. 200 mg
  3. 1800 mg
  4. 2000 mg

Correct Answer: 1800 mg

Solution:

To prepare a 1:10 dilution, you need to add 1800 mg of diluent to 200 mg of plasmid DNA.

If a biotechnologist has 200 mg of a plasmid DNA and needs to prepare a 1:10 dil

Practice Questions

Q1
If a biotechnologist has 200 mg of a plasmid DNA and needs to prepare a 1:10 dilution, how much diluent should be added?
  1. 180 mg
  2. 200 mg
  3. 1800 mg
  4. 2000 mg

Questions & Step-by-Step Solutions

If a biotechnologist has 200 mg of a plasmid DNA and needs to prepare a 1:10 dilution, how much diluent should be added?
  • Step 1: Understand that a 1:10 dilution means you want 1 part of the plasmid DNA and 9 parts of the diluent.
  • Step 2: You have 200 mg of plasmid DNA, which is the 1 part.
  • Step 3: To find out how much diluent to add, calculate 9 parts of diluent. Since 1 part is 200 mg, 9 parts will be 9 times 200 mg.
  • Step 4: Calculate 9 times 200 mg: 9 * 200 = 1800 mg.
  • Step 5: Therefore, you need to add 1800 mg of diluent to the 200 mg of plasmid DNA.
  • Dilution Calculation – Understanding how to prepare a dilution by mixing a solute with a solvent.
  • Volume vs. Mass – Recognizing the difference between mass (mg) and volume (mL) in the context of dilutions.
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