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Which type of PCR is used to amplify RNA?

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Question: Which type of PCR is used to amplify RNA?

Options:

  1. Real-time PCR
  2. Reverse transcription PCR
  3. Multiplex PCR
  4. Nested PCR

Correct Answer: Reverse transcription PCR

Solution: 

Reverse transcription PCR (RT-PCR) is used to amplify RNA by first converting it to complementary DNA (cDNA).

Which type of PCR is used to amplify RNA?

Practice Questions

Q1
Which type of PCR is used to amplify RNA?
  1. Real-time PCR
  2. Reverse transcription PCR
  3. Multiplex PCR
  4. Nested PCR

Questions & Step-by-Step Solutions

Which type of PCR is used to amplify RNA?
  • Step 1: Understand that PCR stands for Polymerase Chain Reaction, which is a method used to make many copies of a specific DNA segment.
  • Step 2: Know that RNA is a different type of nucleic acid than DNA.
  • Step 3: Realize that to amplify RNA, we first need to convert it into DNA. This is done using an enzyme called reverse transcriptase.
  • Step 4: The process of converting RNA into DNA is called reverse transcription, and the resulting DNA is known as complementary DNA (cDNA).
  • Step 5: After the RNA is converted to cDNA, regular PCR can be used to amplify this cDNA.
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