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Which type of PCR is used to amplify RNA?

Practice Questions

Q1
Which type of PCR is used to amplify RNA?
  1. Real-time PCR
  2. Reverse transcription PCR
  3. Multiplex PCR
  4. Nested PCR

Questions & Step-by-Step Solutions

Which type of PCR is used to amplify RNA?
  • Step 1: Understand that PCR stands for Polymerase Chain Reaction, which is a method used to make many copies of a specific DNA segment.
  • Step 2: Know that RNA is a different type of nucleic acid than DNA.
  • Step 3: Realize that to amplify RNA, we first need to convert it into DNA. This is done using an enzyme called reverse transcriptase.
  • Step 4: The process of converting RNA into DNA is called reverse transcription, and the resulting DNA is known as complementary DNA (cDNA).
  • Step 5: After the RNA is converted to cDNA, regular PCR can be used to amplify this cDNA.
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