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What is the role of ligase in recombinant DNA technology?

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Question: What is the role of ligase in recombinant DNA technology?

Options:

  1. To cut DNA strands
  2. To join DNA fragments together
  3. To replicate DNA
  4. To transcribe RNA

Correct Answer: To join DNA fragments together

Solution: 

Ligase is used to join DNA fragments together after they have been cut by restriction enzymes.

What is the role of ligase in recombinant DNA technology?

Practice Questions

Q1
What is the role of ligase in recombinant DNA technology?
  1. To cut DNA strands
  2. To join DNA fragments together
  3. To replicate DNA
  4. To transcribe RNA

Questions & Step-by-Step Solutions

What is the role of ligase in recombinant DNA technology?
  • Step 1: Understand that DNA is made up of smaller pieces called fragments.
  • Step 2: Learn that restriction enzymes are special proteins that cut DNA at specific locations, creating these fragments.
  • Step 3: Recognize that after cutting, we may want to join these DNA fragments together to create a new piece of DNA.
  • Step 4: Know that ligase is another type of enzyme that helps to connect or 'glue' these DNA fragments together.
  • Step 5: Conclude that in recombinant DNA technology, ligase is essential for combining different DNA pieces into a single, functional DNA molecule.
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