Question: How are monoclonal antibodies typically produced?
Options:
By recombinant DNA technology
By chemical synthesis
By fermentation
By natural extraction
Correct Answer: By recombinant DNA technology
Solution:
Monoclonal antibodies are typically produced using recombinant DNA technology.
How are monoclonal antibodies typically produced?
Practice Questions
Q1
How are monoclonal antibodies typically produced?
By recombinant DNA technology
By chemical synthesis
By fermentation
By natural extraction
Questions & Step-by-Step Solutions
How are monoclonal antibodies typically produced?
Step 1: Identify the specific antigen that you want to target with the monoclonal antibody.
Step 2: Inject the antigen into a mouse (or another suitable animal) to stimulate an immune response.
Step 3: After a few weeks, collect the spleen cells from the mouse, which contain the immune cells that produce antibodies.
Step 4: Fuse the spleen cells with myeloma cells (cancer cells that can grow indefinitely) to create hybridoma cells.
Step 5: Screen the hybridoma cells to find those that produce the desired antibody against the antigen.
Step 6: Clone the selected hybridoma cells to produce many identical cells, all producing the same antibody.
Step 7: Harvest the antibodies produced by the cloned hybridoma cells for use in research or therapy.
Monoclonal Antibodies Production – Monoclonal antibodies are produced by creating identical copies of a specific immune cell that produces a desired antibody, often using hybridoma technology or recombinant DNA technology.
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