Step 1: Identify the specific antigen that you want to target with the monoclonal antibody.
Step 2: Inject the antigen into a mouse (or another suitable animal) to stimulate an immune response.
Step 3: After a few weeks, collect the spleen cells from the mouse, which contain the immune cells that produce antibodies.
Step 4: Fuse the spleen cells with myeloma cells (cancer cells that can grow indefinitely) to create hybridoma cells.
Step 5: Screen the hybridoma cells to find those that produce the desired antibody against the antigen.
Step 6: Clone the selected hybridoma cells to produce many identical cells, all producing the same antibody.
Step 7: Harvest the antibodies produced by the cloned hybridoma cells for use in research or therapy.
Monoclonal Antibodies Production – Monoclonal antibodies are produced by creating identical copies of a specific immune cell that produces a desired antibody, often using hybridoma technology or recombinant DNA technology.
