Which enzyme is commonly used to insert DNA fragments into cloning vectors?

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Question: Which enzyme is commonly used to insert DNA fragments into cloning vectors?

Options:

Correct Answer: Ligase

Solution:

DNA ligase is commonly used to insert DNA fragments into cloning vectors by joining the DNA ends.

Which enzyme is commonly used to insert DNA fragments into cloning vectors?

Which enzyme is commonly used to insert DNA fragments into cloning vectors?

Step 1: Understand what a cloning vector is. A cloning vector is a small piece of DNA that can carry foreign DNA into a host cell.
Step 2: Know that DNA fragments are pieces of DNA that you want to insert into the cloning vector.
Step 3: Learn about enzymes. Enzymes are proteins that help speed up chemical reactions in the body.
Step 4: Identify the specific enzyme used for joining DNA pieces. The enzyme used to insert DNA fragments into cloning vectors is called DNA ligase.
Step 5: Understand the function of DNA ligase. DNA ligase works by joining the ends of the DNA fragments together, creating a continuous strand.

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