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What is the function of trypsin in cell culture?

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Question: What is the function of trypsin in cell culture?

Options:

  1. To promote cell growth
  2. To detach adherent cells from the culture surface
  3. To provide nutrients
  4. To buffer the medium

Correct Answer: To detach adherent cells from the culture surface

Solution: 

Trypsin is an enzyme used to detach adherent cells from the culture surface, allowing for passaging and subculturing.

What is the function of trypsin in cell culture?

Practice Questions

Q1
What is the function of trypsin in cell culture?
  1. To promote cell growth
  2. To detach adherent cells from the culture surface
  3. To provide nutrients
  4. To buffer the medium

Questions & Step-by-Step Solutions

What is the function of trypsin in cell culture?
  • Step 1: Understand that trypsin is an enzyme, which means it helps break down proteins.
  • Step 2: In cell culture, cells often stick to the surface of a dish or flask.
  • Step 3: When you want to move these cells to a new dish or flask, you need to detach them.
  • Step 4: Trypsin is added to the dish to break down the proteins that help the cells stick.
  • Step 5: After a short time, the cells become detached from the surface.
  • Step 6: Once the cells are detached, they can be collected and transferred to a new culture dish.
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