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What is the role of reverse transcriptase in recombinant DNA technology?

Practice Questions

Q1
What is the role of reverse transcriptase in recombinant DNA technology?
  1. To synthesize DNA from RNA
  2. To cut DNA at specific sites
  3. To ligate DNA fragments
  4. To amplify DNA sequences

Questions & Step-by-Step Solutions

What is the role of reverse transcriptase in recombinant DNA technology?
  • Step 1: Understand that reverse transcriptase is an enzyme, which is a type of protein that helps speed up chemical reactions in the body.
  • Step 2: Learn that reverse transcriptase's main job is to convert RNA (a type of genetic material) into DNA.
  • Step 3: Recognize that in recombinant DNA technology, scientists often want to work with DNA, not RNA.
  • Step 4: Know that complementary DNA (cDNA) is made from RNA using reverse transcriptase.
  • Step 5: Realize that creating cDNA is important because it allows scientists to study genes and produce proteins in a lab setting.
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