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What is the purpose of using a selectable marker in recombinant DNA technology?

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Question: What is the purpose of using a selectable marker in recombinant DNA technology?

Options:

  1. To enhance protein expression
  2. To identify successfully transformed cells
  3. To increase DNA stability
  4. To reduce mutation rates

Correct Answer: To identify successfully transformed cells

Solution: 

Selectable markers are used to identify successfully transformed cells that have taken up the recombinant DNA.

What is the purpose of using a selectable marker in recombinant DNA technology?

Practice Questions

Q1
What is the purpose of using a selectable marker in recombinant DNA technology?
  1. To enhance protein expression
  2. To identify successfully transformed cells
  3. To increase DNA stability
  4. To reduce mutation rates

Questions & Step-by-Step Solutions

What is the purpose of using a selectable marker in recombinant DNA technology?
  • Step 1: Understand that recombinant DNA technology involves inserting a piece of DNA into a cell.
  • Step 2: Know that not all cells will take up the new DNA; some will remain unchanged.
  • Step 3: A selectable marker is a gene that helps identify which cells have successfully taken up the new DNA.
  • Step 4: The selectable marker usually provides a trait, like antibiotic resistance, that allows only the transformed cells to survive in a specific environment.
  • Step 5: By applying the specific condition (like an antibiotic), only the cells with the selectable marker will grow, making it easy to identify them.
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