What is the main advantage of using high-performance liquid chromatography (HPLC
Practice Questions
Q1
What is the main advantage of using high-performance liquid chromatography (HPLC) for protein purification?
It is less expensive
It provides high resolution and speed
It requires less sample
It is easier to set up
Questions & Step-by-Step Solutions
What is the main advantage of using high-performance liquid chromatography (HPLC) for protein purification?
Step 1: Understand that HPLC stands for High-Performance Liquid Chromatography.
Step 2: Recognize that HPLC is a technique used to separate and purify proteins from a mixture.
Step 3: Learn that the main advantage of HPLC is its ability to provide high resolution, meaning it can separate proteins that are very similar to each other.
Step 4: Note that HPLC also works quickly, allowing for faster purification compared to other methods.
Step 5: Conclude that because of its high resolution and speed, HPLC is a powerful tool for purifying proteins effectively.
