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What is the role of affinity chromatography in protein purification?

Practice Questions

Q1
What is the role of affinity chromatography in protein purification?
  1. To separate proteins based on size
  2. To separate proteins based on charge
  3. To isolate proteins that bind to specific ligands
  4. To denature proteins for analysis

Questions & Step-by-Step Solutions

What is the role of affinity chromatography in protein purification?
  • Step 1: Understand that proteins can bind to specific molecules called ligands.
  • Step 2: Prepare a column filled with a material that has these ligands attached.
  • Step 3: Mix your protein solution with the column so that proteins that bind to the ligands stick to the column.
  • Step 4: Wash the column to remove any proteins that do not bind to the ligands.
  • Step 5: Elute (remove) the bound proteins by adding a solution that disrupts the binding, allowing you to collect the purified proteins.
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