Which technique is commonly used for the initial step in protein purification?
Practice Questions
Q1
Which technique is commonly used for the initial step in protein purification?
Dialysis
Precipitation
Chromatography
Electrophoresis
Questions & Step-by-Step Solutions
Which technique is commonly used for the initial step in protein purification?
Step 1: Understand that protein purification is the process of isolating proteins from a mixture.
Step 2: Know that the first step in this process is often to concentrate the proteins and remove unwanted substances.
Step 3: Learn that precipitation is a common technique used for this initial step.
Step 4: Realize that precipitation involves adding a substance (like salt) to the protein mixture, which causes proteins to clump together and separate from the liquid.
Step 5: Understand that after precipitation, the proteins can be collected and further purified if needed.
